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Characterization of porcine corneal endothelium for xenotransplantation. Semin Ophthalmol 2014 May;29(3):127-35

Date

06/14/2013

Pubmed ID

23758340

DOI

10.3109/08820538.2013.787104

Scopus ID

2-s2.0-84908483152   13 Citations

Abstract

PURPOSE: Endothelial keratoplasty (EKP) has become increasingly popular in the treatment of corneal disease. However, the global shortage of human donor corneas limits clinical corneal transplantation. Genetically engineered (GE) pigs may provide an alternative source of corneas for EKP. The aim of this study was to evaluate corneal endothelial cells (CECs) from wild-type (WT) and GE pigs.

METHODS: Density, size of CECs, and the percentage of hexagonal cells (as a measure of heterogeneity) were measured by ex vivo confocal microscopy in corneas from WT and GE pigs of different ages - neonatal (4-5 days), young (5-15 weeks), adult (5-15 months), and old (20-42 months). I?1,3-galactosyltransferase gene-knockout (GTKO) pigs transgenic for the human complement-regulatory protein(s), CD46 (GTKO/CD46) +/- CD55 (GTKO/CD46/CD55) were used as sources of GE corneas.

RESULTS: Mean CEC densities (cells/mmA?) were neonatal (5968), young (3789), adult (2589), and old (2070). As with human corneas, there was an age-dependent decrease in pig CEC density and increase in pig CEC size. However, unlike human corneas, there was no correlation between the percentage of hexagonal cells (approximately 50% in all pig corneas) and age, suggesting that heterogeneity is intrinsic for pig corneas. Genetic modification did not affect CEC density, size, or morphology compared to WT pigs.

CONCLUSION: Because of the availability of young pigs and their greater CEC density (and the protection afforded against the human immune response), GE pigs could provide an unlimited source of corneas for clinical EKP.

Author List

Lee SE, Mehra R, Fujita M, Roh DS, Long C, Lee W, Funderburgh JL, Ayares DL, Cooper DK, Hara H

Author

Whayoung Lee MD Assistant Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Genetically Modified
CD55 Antigens
Cell Count
Cell Size
Corneal Transplantation
Endothelium, Corneal
Galactosyltransferases
Gene Knockout Techniques
Macaca mulatta
Membrane Cofactor Protein
Microscopy, Confocal
Swine
Transplantation, Heterologous