Faculty Collaboration Database

Spontaneous, persistent infection of a B-cell lymphoma with adenovirus. J Med Virol 1996 Mar;48(3):267-72

Date

03/01/1996

Pubmed ID

8801288

DOI

10.1002/(SICI)1096-9071(199603)48:3<267::AID-JMV9>3.0.CO;2-6

Scopus ID

2-s2.0-0029985920 (requires institutional sign-in at Scopus site)   31 Citations

Abstract

An adenovirus culture-positive lymphoblastoid cell line was derived from a bone marrow transplant recipient with fatal B-cell lymphoproliferative disease and adenovirus pneumonia. At autopsy, focal areas of the lymphoma infiltrating the patient's lung were positive for adenovirus proteins by immunohistochemical staining. The Epstein-Barr virus-transformed B-cell line Mk, established from pleural fluid cells, contained adenovirus virions in both the nucleus and the cytoplasm by electron microscopy. The majority of Mk cells expressed adenovirus proteins and produced a high level of infectious adenovirus by plaque assay analysis. However, in contrast to the rapid cell death induced by adenovirus in other permissive cell lines, Mk was maintained stably in tissue culture for 6 months. These data indicate that adenoviral replication is not sufficient for cell lysis and confirm that adenovirus can cause persistent infection in human lymphoid cells in vivo.

Author List

Flomenberg P, Piaskowski V, Harb J, Segura A, Casper JT

Author

Annette D. Segura MD Adjunct Assistant Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adenoviridae Infections
Adenoviruses, Human
Bone Marrow
Bone Marrow Cells
Bone Marrow Transplantation
Capsid
Capsid Proteins
Child, Preschool
Herpesvirus 4, Human
Humans
Lymphoma, B-Cell
Male
Pneumonia, Viral
Tumor Cells, Cultured
Virus Latency