Faculty Collaboration Database

Identification of virus resistant tumor cell subpopulations in three-dimensional uveal melanoma cultures. Cancer Gene Ther 2010 Apr;17(4):223-34

Date

11/07/2009

Pubmed ID

19893596

DOI

10.1038/cgt.2009.73

Scopus ID

2-s2.0-77949654118 (requires institutional sign-in at Scopus site)   16 Citations

Abstract

To better understand melanoma resistance to herpes simplex virus type 1 (HSV-1)-mediated oncolysis, traditional two-dimensional (2D) cultures and extracellular matrix (ECM) containing three-dimensional (3D) cultures of OCM1 and C918 uveal melanoma cells were infected with an HSV-1 strain that expresses the green fluorescent protein (GFP) marker during replication. Although 2D cultures were completely destroyed within a few days of HSV-1 inoculation, viable GFP-negative tumor cells remained detectable in 3D cultures for several weeks. Tumor cells with increased resistance to HSV-1 included cells that formed vasculogenic mimicry patterns and multicellular spheroids and cells that invaded Matrigel individually. Mechanisms of tumor resistance against HSV-1 in the 3D environment included impaired virus spread in the ECM and ECM-mediated inhibition of viral replication after viral entry into tumor cells. Observations also suggested that HSV-1 established quiescent infection in some tumor cells present in multicellular spheroids and that this could revert to productive viral infection when the tumor growth pattern changed. These findings indicate that 3D tumor cell cultures can be used to identify distinct tumor cell populations with increased resistance to HSV-1 and to explore mechanisms of ECM-mediated tumor resistance to oncolytic virotherapy.

Author List

Valyi-Nagy K, Dosa S, Kovacs SK, Bacsa S, Voros A, Shukla D, Folberg R, Valyi-Nagy T

Author

Sandor K. Kovacs MD Assistant Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Cell Culture Techniques
Collagen
Drug Combinations
Drug Resistance, Viral
Extracellular Matrix
Herpesvirus 1, Human
Humans
Laminin
Melanoma
Oncolytic Virotherapy
Proteoglycans
Tumor Cells, Cultured
Uveal Neoplasms
Virus Replication