Faculty Collaboration Database

Identification of 45 kD antigen in immune complexes of patients of allergic bronchopulmonary aspergillosis. Mol Cell Biochem 1997 Jan;166(1-2):111-6

Date

01/01/1997

Pubmed ID

9046027

DOI

10.1023/a:1006827126958

Scopus ID

2-s2.0-0031059481 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

A glycoprotein antigen with an apparent mw of 45 kD was observed to be predominant in the circulating immune complexes isolated from patients of allergic bronchopulmonary aspergillosis as well as in the immune complexes prepared in vitro. Further characterisation of 45 kD antigen with trypsin showed four immunologically active peptides with mw 43, 36, 33 and 16 kD. Carbohydrate characterization using various lectins (Maackia amurensis agglutinin, Sambucus nigra agglutinin, Peanut agglutinin, Galanthus nivalis agglutinin and Datura stramonium agglutinin) showed presence of both N-linked and O-linked sugar moieties such as mannose, glucose, galactose and N-acetyl glucosamine. Predominant presence of 45 kD antigen in immune complexes, recognition of 45 kD by monoclonal antibodies raised against glycoprotein rich fraction of A. fumigatus and presence of elastinolytic protease activity indicate that 45 kD antigen is probably a potent virulence factor and may be contributing to the pathogenesis of ABPA by its biological as well as immunological activities.

Author List

Madan T, Banerjee B, Bhatnagar PK, Shah A, Sarma PU

Author

Banani Banerjee PhD Associate Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Antigen-Antibody Complex
Antigens, Fungal
Aspergillosis, Allergic Bronchopulmonary
Female
Galanthus
Humans
Lectins
Male
Molecular Weight
Plant Lectins
Trypsin