Faculty Collaboration Database

Transcellular transport is not required for transmucosal bacterial passage across the intestinal membrane ex vivo. Surg Infect (Larchmt) 2000;1(4):265-72

Date

02/22/2003

Pubmed ID

12594882

DOI

10.1089/109629600750067192

Scopus ID

2-s2.0-0041659626 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

The mechanisms underlying the process of bacterial translocation are poorly defined. Possible routes for transmucosal passage of bacteria include transcellular and paracellular channels. Bacterial engulfment is a prerequisite for transcellular transport. To determine whether transcellular transport is required for transmucosal bacterial passage, we examined the effect of various inhibitors of endocytosis, such as colchicine, cytochalasin B, and sodium fluoride on transmucosal passage of bacteria across an ileal mucosal membrane mounted in the Ussing chamber. Colchicine and sodium fluoride increased the rate of decline of the potential difference across the membranes. However, neither colchicine, cytochalasin B, nor sodium fluoride affected the incidence of transmucosal bacterial passage. Sodium fluoride, which depletes intracellular ATP, significantly decreased the number of bacteria that passed per membrane. Our data suggest that transcellular transport may not be required for spontaneous transmucosal passage of bacteria, and furthermore bacterial passage may be, at least in part, an energy-dependent process.

Author List

Nadler EP, Go LL, Beer-Stolz D, Watkins SC, Schall LC, Boyle P, Ford HR

Author

Laura Cassidy PhD Associate Dean, Professor in the Institute for Health and Equity department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Bacterial Translocation
Colchicine
Cytochalasin B
Endocytosis
Energy Metabolism
Escherichia coli
Ileum
In Vitro Techniques
Intestinal Mucosa
Male
Rats
Rats, Sprague-Dawley
Sodium Fluoride