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Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. Methods Mol Biol 2023;2599:177-190

Date

11/26/2022

Pubmed ID

36427150

DOI

10.1007/978-1-0716-2847-8_13

Scopus ID

2-s2.0-85142940540 (requires institutional sign-in at Scopus site)   1 Citation

Abstract

Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions. Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions. Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.

Author List

Hammonds EF, Morrison EA

Author

Emma A. Morrison PhD Assistant Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

DNA
Histones
Humans
Nucleosomes
Sucrose