Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. Methods Mol Biol 2023;2599:177-190
Date
11/26/2022Pubmed ID
36427150DOI
10.1007/978-1-0716-2847-8_13Scopus ID
2-s2.0-85142940540 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions. Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions. Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.
Author List
Hammonds EF, Morrison EAAuthor
Emma A. Morrison PhD Assistant Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
DNAHistones
Humans
Nucleosomes
Sucrose