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Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans. Biochem J 1995 Aug 15;310 ( Pt 1)(Pt 1):311-4

Date

08/15/1995

Pubmed ID

7646461

Pubmed Central ID

PMC1135889

DOI

10.1042/bj3100311

Scopus ID

2-s2.0-0029155341 (requires institutional sign-in at Scopus site)   21 Citations

Abstract

EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

Author List

Sears HJ, Bennett B, Spiro S, Thomson AJ, Richardson DJ

Author

Brian Bennett D.Phil. Professor and Chair in the Physics department at Marquette University




MESH terms used to index this publication - Major topics in bold

Cold Temperature
Electron Spin Resonance Spectroscopy
Nitrate Reductase
Nitrate Reductases
Paracoccus denitrificans