Faculty Collaboration Database

Arginine vasopressin-mediated stimulation of nitric oxide within the rat renal medulla. Hypertension 1998 Nov;32(5):896-901

Date

11/20/1998

Pubmed ID

9822450

DOI

10.1161/01.hyp.32.5.896

Scopus ID

2-s2.0-0031738947 (requires institutional sign-in at Scopus site)   52 Citations

Abstract

The present study was designed to determine whether arginine vasopressin (AVP) can stimulate nitric oxide (NO) production within the renal medulla and thereby modulate renal medullary blood flow. An in vivo microdialysis/NO trapping technique was used to determine changes in medullary interstitial [NO]. AVP (2 ng/kg per minute) was delivered into the renal medullary interstitium and resulted in a significant increase in renal medullary [NO] of 35%, which was blocked by pretreatment with nitro-L-arginine methyl ester (L-NAME) (1.3 microg/kg per minute) administered into the renal medullary interstitium. The vasopressin V2 receptor agonist 1-desamino-8-D-arginine vasopressin (dDAVP) resulted in a significant increase of 32% in renal medullary interstitial [NO]. No change in renal medullary interstitial [NO] was observed after selective vasopressin V1 receptor stimulation. Laser-Doppler flowmetry with implanted optical fibers was performed to measure cortical and medullary blood flow changes within the kidney. Renal interstitial infusion of dDAVP in rats pretreated with a vasopressin V1 receptor antagonist resulted in a 15% increase (P<0.05) in medullary blood flow, which was completely blocked by pretreatment with L-NAME (1.3 microg/kg per minute). This study demonstrates that AVP increases renal medullary interstitial [NO] through vasopressin V2 receptor stimulation, which in turn elevates blood flow to the renal medulla.

Author List

Park F, Zou AP, Cowley AW Jr

Author

Allen W. Cowley Jr PhD Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Arginine Vasopressin
Enzyme Inhibitors
Kidney Medulla
Male
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Rats
Rats, Sprague-Dawley
Receptors, Vasopressin
Renal Agents