Faculty Collaboration Database

Precision immunointerception of EGFR-driven tumorigenesis for lung cancer prevention. Front Immunol 2023;14:1036563

Date

03/07/2023

Pubmed ID

36875137

Pubmed Central ID

PMC9982083

DOI

10.3389/fimmu.2023.1036563

Scopus ID

2-s2.0-85149504680 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

Epidermal growth factor receptor (EGFR) mutations occur in about 50% of lung adenocarcinomas in Asia and about 15% in the US. EGFR mutation-specific inhibitors have been developed and made significant contributions to controlling EGFR mutated non-small cell lung cancer. However, resistance frequently develops within 1 to 2 years due to acquired mutations. No effective approaches that target mutant EGFR have been developed to treat relapse following tyrosine kinase inhibitor (TKI) treatment. Vaccination against mutant EGFR is one area of active exploration. In this study, we identified immunogenic epitopes for the common EGFR mutations in humans and formulated a multi-peptide vaccine (E^mut Vax) targeting the EGFR L858R, T790M, and Del19 mutations. The efficacy of the E^mut Vax was evaluated in both syngeneic and genetic engineered EGFR mutation-driven murine lung tumor models with prophylactic settings, where the vaccinations were given before the onset of the tumor induction. The multi-peptide E^mut Vax effectively prevented the onset of EGFR mutation-driven lung tumorigenesis in both syngeneic and genetically engineered mouse models (GEMMs). Flow cytometry and single-cell RNA sequencing were conducted to investigate the impact of E^mut Vax on immune modulation. E^mut Vax significantly enhanced Th1 responses in the tumor microenvironment and decreased suppressive Tregs to enhance anti-tumor efficacy. Our results show that multi-peptide E^mut Vax is effective in preventing common EGFR mutation-driven lung tumorigenesis, and the vaccine elicits broad immune responses that are not limited to anti-tumor Th1 response.

Author List

Pan J, Xiong D, Zhang Q, Palen K, Shoemaker RH, Johnson B, Sei S, Wang Y, You M

Author

Bryon D. Johnson PhD Adjunct Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Carcinogenesis
Carcinoma, Non-Small-Cell Lung
Cell Transformation, Neoplastic
ErbB Receptors
Humans
Lung Neoplasms
Mice
Mutation
Neoplasm Recurrence, Local
Protein Kinase Inhibitors
Tumor Microenvironment