Faculty Collaboration Database

Cultured cardiac fibroblasts and myofibroblasts express Sushi Containing Domain 2 and assemble a unique fibronectin rich matrix. Exp Cell Res 2021 Feb 15;399(2):112489

Date

01/17/2021

Pubmed ID

33453237

DOI

10.1016/j.yexcr.2021.112489

Scopus ID

2-s2.0-85100124352 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

Cardiac fibroblasts and myofibroblasts assemble and maintain extracellular matrix during normal development and following injury. Culture expansion of these cells yield a bioengineered matrix that could lead to intriguing therapeutic opportunities. For example, we reported that cultured rat cardiac fibroblasts form a matrix that can be used to delivery therapeutic stem cells. Furthermore, we reported that matrix derived from cultured human cardiac fibroblasts/myofibroblasts converted monocytes into macrophages that express interesting anti-inflammatory and pro-angiogenic properties. Expanding these matrix investigations require characterization of the source cells for quality control. In these efforts, we observed and herein report that Sushi Containing Domain 2 (SUSD2) is a novel and consistent marker for cultured human cardiac fibroblast and myofibroblasts.

Author List

Schmuck EG, Roy S, Dhillon A, Walker S, Spinali K, Colevas S, Zhou T, Chhabra G, Liu Y, Kader Sagar MA, Childs CJ, Kink JA, Eliceiri KW, Hematti P, Raval AN

Author

Peiman Hematti MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Biomarkers
Cells, Cultured
Extracellular Matrix
Female
Fibroblasts
Fibronectins
Humans
Male
Membrane Glycoproteins
Myocardium
Myofibroblasts