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Cellular inflammatory infiltrate in pneumonitis induced by a single moderate dose of thoracic x radiation in rats. Radiat Res 2010 Apr;173(4):545-56

Date

03/26/2010

Pubmed ID

20334527

Pubmed Central ID

PMC2904484

DOI

10.1667/RR1753.1

Scopus ID

2-s2.0-77950256999 (requires institutional sign-in at Scopus site)   39 Citations

Abstract

The goal of these studies was to characterize the infiltrating inflammatory cells during pneumonitis caused by moderate doses of radiation. Two groups of male rats (WAG/RijCmcr, 8 weeks old) were treated with single 10- or 15-Gy doses of thoracic X radiation; a third group of age-matched animals served as controls. Only 25% rats survived the 15-Gy dose. Bronchoalveolar lavage fluid and whole lung mounts were subjected to cytological and histological evaluation after 8 weeks for distribution of resident macrophages, neutrophils, lymphocytes and mast cells. There was a modest increase in airway and airspace-associated neutrophils in lungs from rats receiving 15 Gy. Mast cells (detected by immunohistochemistry for tryptase) increased over 70% with 10 Gy and over 13-fold after 15 Gy, with considerable leakage of tryptase into blood vessels and airways. Circulating levels of eight inflammatory cytokines were not altered after 10 Gy but appeared to decrease after 15 Gy. In summary, there were only modest increases in cellular inflammatory infiltrate during pneumonitis after a non-lethal dose of 10 Gy, but there was a dramatic rise in mast cell infiltration after 15 Gy, suggesting that circulating levels of mast cell products may be useful markers of severe pneumonitis.

Author List

Szabo S, Ghosh SN, Fish BL, Bodiga S, Tomic R, Kumar G, Morrow NV, Moulder JE, Jacobs ER, Medhora M

Author

Natalya V. Morrow PhD Assistant Professor in the Radiation Oncology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cytokines
Dose-Response Relationship, Radiation
Lung
Male
Radiation Dosage
Radiation Pneumonitis
Radiography, Thoracic
Rats
Rats, Wistar
Survival Rate