Protocol to generate cardiac pericytes from human induced pluripotent stem cells. STAR Protoc 2023 Apr 27;4(2):102256
Date
04/29/2023Pubmed ID
37119139Pubmed Central ID
PMC10173856DOI
10.1016/j.xpro.2023.102256Scopus ID
2-s2.0-85153537578 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Cardiac pericytes are a critical yet enigmatic cell type within the coronary microvasculature. Since primary human cardiac pericytes are not readily accessible, we present a protocol to generate them from human induced pluripotent stem cells (hiPSCs). Our protocol involves several steps, including the generation of intermediate cell types such as mid-primitive streak, lateral plate mesoderm, splanchnic mesoderm, septum transversum, and epicardium, before deriving cardiac pericytes. With hiPSC-derived cardiac pericytes, researchers can decipher the mechanisms underlying coronary microvascular dysfunction. For complete details on the use and execution of this protocol, please refer to Shen et al.1.