Faculty Collaboration Database

Protocol to generate cardiac pericytes from human induced pluripotent stem cells. STAR Protoc 2023 Apr 27;4(2):102256

Date

04/29/2023

Pubmed ID

37119139

Pubmed Central ID

PMC10173856

DOI

10.1016/j.xpro.2023.102256

Scopus ID

2-s2.0-85153537578 (requires institutional sign-in at Scopus site)

Abstract

Cardiac pericytes are a critical yet enigmatic cell type within the coronary microvasculature. Since primary human cardiac pericytes are not readily accessible, we present a protocol to generate them from human induced pluripotent stem cells (hiPSCs). Our protocol involves several steps, including the generation of intermediate cell types such as mid-primitive streak, lateral plate mesoderm, splanchnic mesoderm, septum transversum, and epicardium, before deriving cardiac pericytes. With hiPSC-derived cardiac pericytes, researchers can decipher the mechanisms underlying coronary microvascular dysfunction. For complete details on the use and execution of this protocol, please refer to Shen et al.¹.

Author List

Shen M, Zhao SR, Khokhar Y, Li L, Zhou Y, Liu C, Wu JC

Author

Chun Liu PhD Assistant Professor in the Physiology department at Medical College of Wisconsin