Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study. J Pediatr 2022 Feb;241:251-256.e4
Date
10/10/2021Pubmed ID
34626671Pubmed Central ID
PMC8838877DOI
10.1016/j.jpeds.2021.09.061Scopus ID
2-s2.0-85117910103 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Newborn screening using dried plasma spots offers preanalytical advantages over conventional cards for plasma-associated targets of interest. Herein we present dried plasma spot-based methods for measuring metabolites using a 250+ compound liquid chromatography tandem mass spectrometry library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.
Author List
Schleif WS, Harlan RS, Hamblin F, Amankwah EK, Goldenberg NA, Hernandez RG, Johnson SB, Reed S, Graham DRAuthor
Ernest Amankwah PhD Director, Associate Professor in the Clinical and Translational Science Institute department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BiomarkersBlood Preservation
Chromatography, Liquid
Dried Blood Spot Testing
Female
Humans
Infant
Infant, Newborn
Male
Metabolome
Neonatal Screening
Prospective Studies
Reference Values
Specimen Handling
Tandem Mass Spectrometry