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Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study. J Pediatr 2022 Feb;241:251-256.e4

Date

10/10/2021

Pubmed ID

34626671

Pubmed Central ID

PMC8838877

DOI

10.1016/j.jpeds.2021.09.061

Scopus ID

2-s2.0-85117910103 (requires institutional sign-in at Scopus site)   1 Citation

Abstract

Newborn screening using dried plasma spots offers preanalytical advantages over conventional cards for plasma-associated targets of interest. Herein we present dried plasma spot-based methods for measuring metabolites using a 250+ compound liquid chromatography tandem mass spectrometry library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.

Author List

Schleif WS, Harlan RS, Hamblin F, Amankwah EK, Goldenberg NA, Hernandez RG, Johnson SB, Reed S, Graham DR

Author

Ernest Amankwah PhD Director, Associate Professor in the Clinical and Translational Science Institute department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Biomarkers
Blood Preservation
Chromatography, Liquid
Dried Blood Spot Testing
Female
Humans
Infant
Infant, Newborn
Male
Metabolome
Neonatal Screening
Prospective Studies
Reference Values
Specimen Handling
Tandem Mass Spectrometry