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Sprayable and injectable visible-light Kappa-carrageenan hydrogel for in-situ soft tissue engineering. Int J Biol Macromol 2019 Oct 01;138:590-601

Date

07/26/2019

Pubmed ID

31344417

DOI

10.1016/j.ijbiomac.2019.07.126

Scopus ID

2-s2.0-85069851861 (requires institutional sign-in at Scopus site)   72 Citations

Abstract

The aim of this study was to develop injectable and sprayable visible-light crosslinked Kappa-carrageenan (κCA) hydrogel and to investigate the role of polymer concentration (2, 4 and 6 wt%) and degree of methacrylation (6 and 12%) on its properties. It was found that, the average pore sizes, water content and swelling ratio of hydrogel were tunable by changing the methacrylate κCA (KaMA) concentration and methacrylation degree. Furthermore, the mechanical properties of KaMA could be noticeably modulated, depending on the formulation of hydrogel. Tensile and comprehensive modules were enhanced from 68 to 357 kPa and from 213 to 357 kPa, respectively, by increasing KaMA concentration from 2 to 6 wt% and methacrylation degree from 6 to 12%. Furthermore, with increasing methacrylation degree and polymer content, the absorbed energy and energy loss were increased. Moreover, recovery significantly enhanced from 27.3% to 74.4% with increasing polymer content from 2 to 6 wt%. Finally, visible-light crosslinked KaMA hydrogels not only was biocompatible, but also could promote HaLa cell and fibloblasts function. The visible-light crosslinked KaMA is thought to be an exclusive biomaterial as a sprayable hydrogel being able to cover skin injuries or to inject as a bio-printing material to in situ heal soft tissue damages.

Author List

Tavakoli S, Kharaziha M, Kermanpur A, Mokhtari H

Author

Samon Tavakoli MD Instructor in the Neurosurgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adsorption
Biocompatible Materials
Carrageenan
Cell Line
Chemical Phenomena
Connective Tissue
Cross-Linking Reagents
Humans
Hydrogels
Light
Materials Testing
Mechanical Phenomena
Tissue Engineering