DNA flow cytometry of sebaceous cell carcinomas of the ocular adnexa: introduction to the technique in the evaluation of periocular tumors. Ophthalmic Plast Reconstr Surg 1992;8(2):77-87
Date
01/01/1992Pubmed ID
1520661DOI
10.1097/00002341-199206000-00001Scopus ID
2-s2.0-0026649816 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
DNA content abnormalities are well recognized in tumor cell biology and kinetics. We introduce the technique of DNA flow cytometry through the study of the sebaceous cell carcinomas of the ocular adnexa. By correlating the data to the standard histopathologic parameters of pagetoid changes, degree of anaplasia, and stromal inflammation, significant associations are revealed. All aneuploid tumors demonstrate pagetoid spread, and more severe anaplasia and stromal inflammation. All diploid tumors are nonpagetoid and have lesser degrees of anaplasia and stromal inflammation. A complete review of the technique with a discussion of the implications and applicability to the study of ocular adnexal tumors is presented.
Author List
Sakol PJ, Simons KB, McFadden PW, Harris GJ, Massaro BM, Koethe SAuthor
Gerald J. Harris MD Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AgedAged, 80 and over
Aneuploidy
Carcinoma
DNA, Neoplasm
Diploidy
Eyelid Neoplasms
Female
Flow Cytometry
Follow-Up Studies
Humans
Male
Middle Aged
Sebaceous Gland Neoplasms
