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NMR solution structure of plastocyanin from the photosynthetic prokaryote, Prochlorothrix hollandica. Biochemistry 1999 Apr 20;38(16):4988-95

Date

04/23/1999

Pubmed ID

10213601

DOI

10.1021/bi983024f

Scopus ID

2-s2.0-0033586717   32 Citations

Abstract

The solution structure of a divergent plastocyanin (PC) from the photosynthetic prokaryote Prochlorothrix hollandica was determined by homonuclear 1H NMR spectroscopy. Nineteen structures were calculated from 1222 distance restraints, yielding a family of structures having an average rmsd of 0.42 +/- 0.08 A for backbone atoms and 0.71 +/- 0.07 A for heavy atoms to the mean structure. No distance constraint was violated by more than 0.26 A in the structure family. Despite the low number of conserved residues shared with other PC homologues, the overall folding pattern of P. hollandica PC is similar to other PCs, in that the protein forms a two-sheet beta-barrel tertiary structure. The greatest variability among the backbone structures is seen in the loop region from residues 47-60. The differences seen in the P. hollandica PC homologue likely arise due to a small deletion of 2-4 residues compared to the PC consensus; this yields a less extended loop containing a short alpha-helix from residues Ala52-Leu55. Additionally, the protein has an altered hydrophobic patch thought to be important in binding reaction partners. Whereas the backbone structure is very similar within the loops of the hydrophobic region, the presence of two unique residues (Tyr12 and Pro14) yields a structurally different hydrophobic surface likely important in binding P. hollandica Photosystem I.

Author List

Babu CR, Volkman BF, Bullerjahn GS

Author

Brian F. Volkman PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Crystallography, X-Ray
Electron Transport
Hydrogen Bonding
Models, Molecular
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Photosynthesis
Plastocyanin
Prochlorothrix
Proline
Protein Conformation
Protein Structure, Secondary
Protons
Sequence Homology, Amino Acid
Solutions
jenkins-FCD Prod-444 eb4ebd1a08581aba961d3befd3b851a3c3ec6b46