Bradykinin induces superoxide anion release from human endothelial cells. J Cell Physiol 1990 Apr;143(1):21-5
Date
04/01/1990Pubmed ID
2156873DOI
10.1002/jcp.1041430104Scopus ID
2-s2.0-0025306418 (requires institutional sign-in at Scopus site) 134 CitationsAbstract
The time-dependent release of superoxide anion (O2-) from bradykinin (Bk)-stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O2- release by EC was not detectable. EC exposure to Bk (10(-6) to 10(-5) M) resulted in a rapid release of O2-. The release of O2- occurred within 5 minutes of exposure. O2- release was partially inhibited by indomethacin (63 +/- 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O2- production. EC O2- release may be an important component in the pathophysiologic actions of Bk on vascular function.
Author List
Holland JA, Pritchard KA, Pappolla MA, Wolin MS, Rogers NJ, Stemerman MBAuthor
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BradykininCalcimycin
Cells, Cultured
Endothelium, Vascular
Epoprostenol
Humans
In Vitro Techniques
Indomethacin
Prostaglandin-Endoperoxide Synthases
Superoxides