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Bradykinin induces superoxide anion release from human endothelial cells. J Cell Physiol 1990 Apr;143(1):21-5

Date

04/01/1990

Pubmed ID

2156873

DOI

10.1002/jcp.1041430104

Scopus ID

2-s2.0-0025306418   124 Citations

Abstract

The time-dependent release of superoxide anion (O2-) from bradykinin (Bk)-stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry. In the absence of Bk, O2- release by EC was not detectable. EC exposure to Bk (10(-6) to 10(-5) M) resulted in a rapid release of O2-. The release of O2- occurred within 5 minutes of exposure. O2- release was partially inhibited by indomethacin (63 +/- 6%), thus suggesting that arachidonic acid metabolism, through cyclooxygenase, contributes to EC O2- production. EC O2- release may be an important component in the pathophysiologic actions of Bk on vascular function.

Author List

Holland JA, Pritchard KA, Pappolla MA, Wolin MS, Rogers NJ, Stemerman MB

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Bradykinin
Calcimycin
Cells, Cultured
Endothelium, Vascular
Epoprostenol
Humans
In Vitro Techniques
Indomethacin
Prostaglandin-Endoperoxide Synthases
Superoxides
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a