Faculty Collaboration Database

Autofluorescence of treated retinoblastoma. J AAPOS 2011 Apr;15(2):167-72

Date

05/21/2011

Pubmed ID

21596295

DOI

10.1016/j.jaapos.2010.12.011

Scopus ID

2-s2.0-79956227328 (requires institutional sign-in at Scopus site)   12 Citations

Abstract

PURPOSE: To describe the autofluorescent features of retinoblastomas after treatment.

METHODS: Standard fundus photography and autofluorescence (AF) photography (580 nm excitation, 695 nm barrier filter) were performed on 88 tumors of 61 patients. Clinical features were correlated with autofluorescent features.

RESULTS: The mean patient age at AF was 10.3 years. Of the 88 tumors, 5 (6%) were untreated, and 83 (94%) were treated. The untreated retinoblastomas showed hyperautofluorescence (hyperAF) at the site of calcification in all 5 cases (100%). The treated retinoblastomas showed intrinsic calcification in 54 cases (65%) and bright hyperAF at the site of calcification was detected in all cases. Of the 60 tumors with noncalcified remnant, the noncalcified portion was mildly hyperAF in 20 (33%), isoautofluorescence in 31 (52%), and mildly hypoautofluorescence (hypoAF) in 9 (15%). Surrounding retinal pigment epithelium hyperplasia appeared moderately hypoAF in 58 of 58 eyes (100%). Retinal pigment epithelium atrophy appeared mildly hyperAF in 29 (37%), isoautofluorescence in 33 (42%), and mildly hypoAF in 16 (21%).

CONCLUSIONS: AF of retinoblastoma generally shows bright hyperAF of the calcified portion and variable AF of the noncalcified portion. The AF of calcification in retinoblastoma was confirmed by fluorescent microscopy of unstained sections of retinoblastoma after enucleation.

Author List

Ramasubramanian A, Shields CL, Mellen PL, Haji S, Harmon SA, Vemuganti GK, Shields JA

Author

Aparna Ramasubramanian MD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Atrophy
Calcinosis
Child
Child, Preschool
Female
Fluorescein Angiography
Fluorescence
Humans
Hyperplasia
Male
Photography
Retinal Neoplasms
Retinal Pigment Epithelium
Retinoblastoma
Visual Acuity
Young Adult