Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol 2022;2429:233-246
Date
05/05/2022Pubmed ID
35507165Pubmed Central ID
PMC9667909DOI
10.1007/978-1-0716-1979-7_15Scopus ID
2-s2.0-85129368742 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
Vascular smooth muscle cells (VSMCs), a highly mosaic tissue, arise from multiple distinct embryonic origins and populate different regions of our vascular network with defined boundaries. Accumulating evidence has revealed that the heterogeneity of VSMC origins contributes to region-specific vascular diseases such as atherosclerosis and aortic aneurysm. These findings highlight the necessity of taking into account lineage-dependent responses of VSMCs to common vascular risk factors when studying vascular diseases. This chapter describes a reproducible, stepwise protocol for the generation of isogenic VSMC subtypes originated from proepicardium, second heart field, cardiac neural crest, and ventral somite using human induced pluripotent stem cells. By leveraging this robust induction protocol, patient-derived VSMC subtypes of desired embryonic origins can be generated for disease modeling as well as drug screening and development for vasculopathies with regional susceptibility.
Author List
Shen M, Liu C, Wu JCAuthor
Chun Liu PhD Assistant Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AtherosclerosisCell Differentiation
Cells, Cultured
Humans
Induced Pluripotent Stem Cells
Muscle, Smooth, Vascular
Myocytes, Smooth Muscle
