Strain Specific Phage Treatment for Staphylococcus aureus Infection Is Influenced by Host Immunity and Site of Infection. PLoS One 2015;10(4):e0124280
Date
04/25/2015Pubmed ID
25909449Pubmed Central ID
PMC4409319DOI
10.1371/journal.pone.0124280Scopus ID
2-s2.0-84928789019 (requires institutional sign-in at Scopus site) 60 CitationsAbstract
The response to multi-drug resistant bacterial infections must be a global priority. While mounting resistance threatens to create what the World Health Organization has termed a "post-antibiotic era", the recent discovery that antibiotic use may adversely impact the microbiome adds further urgency to the need for new developmental approaches for anti-pathogen treatments. Methicillin-resistant Staphylococcus aureus (MRSA), in particular, has declared itself a serious threat within the United States and abroad. A potential solution to the problem of antibiotic resistance may not entail looking to the future for completely novel treatments, but instead looking into our history of bacteriophage therapy. This study aimed to test the efficacy, safety, and commercial viability of the use of phages to treat Staphylococcus aureus infections using the commercially available phage SATA-8505. We found that SATA-8505 effectively controls S. aureus growth and reduces bacterial viability both in vitro and in a skin infection mouse model. However, this killing effect was not observed when phage was cultured in the presence of human whole blood. SATA-8505 did not induce inflammatory responses in peripheral blood mononuclear cultures. However, phage did induce IFN gamma production in primary human keratinocyte cultures and induced inflammatory responses in our mouse models, particularly in a mouse model of chronic granulomatous disease. Our findings support the potential efficacy of phage therapy, although regulatory and market factors may limit its wider investigation and use.
Author List
Pincus NB, Reckhow JD, Saleem D, Jammeh ML, Datta SK, Myles IAAuthor
Momodou L. Jammeh MD Assistant Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBacteriolysis
Bacteriophages
Disease Models, Animal
Host-Pathogen Interactions
Humans
Keratinocytes
Methicillin-Resistant Staphylococcus aureus
Mice
Microbial Viability
Staphylococcal Infections
Staphylococcus aureus
