Sublytic complement attack reduces infarct size in rabbit isolated hearts: evidence for C5a-mediated cardioprotection. Immunopharmacology 2000 Sep;49(3):391-9
Date
09/21/2000Pubmed ID
10996036DOI
10.1016/s0162-3109(00)00258-7Scopus ID
2-s2.0-0033833623 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Sublytic complement attack can elicit protective cellular responses without precipitating cell death. Our investigation examined the effects of non-lethal complement activation in isolated hearts. New Zealand white rabbit hearts were subjected to 30 min of ischemia followed by 1 h of reperfusion. Prior to ischemia, hearts were perfused for 20 min with 0.5% normal human plasma (NHP). Hearts treated with NHP developed significantly (p<0.05) smaller infarcts compared with controls, expressed as percent of area at risk (AAR) (25.3+/-4.0% vs. 40.9+/-4.3%, respectively). Heat-inactivation, soluble complement receptor 1 (sCR1; 20 nM), and anti-C5a antibody reversed the protective effect of NHP (39.0+/-3.1%, 41.7+/-5.1% and 38.4+/-2.3% AAR, respectively). Hearts treated with 3 nM C5a exhibited infarct sizes similar to those exposed to NHP (27.6+/-5.0% AAR). sCR1 alone did not affect infarct size (37.9+/-4.5% AAR). The results suggest that non-lethal complement activation attenuates reperfusion injury through formation of C5a.
Author List
Tanhehco EJ, Lee H, Lucchesi BRAuthor
Elaine J. Tanhehco MD, PhD Assistant Professor in the Radiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsComplement Activation
Complement C5a
Complement Membrane Attack Complex
Hemodynamics
Hemolysis
Humans
In Vitro Techniques
Male
Myocardial Infarction
Myocardial Reperfusion
Rabbits
Receptors, Complement 3b
Solubility
