Faculty Collaboration Database

Collagenase 1 and collagenase 3 expression in a guinea pig model of osteoarthritis. Arthritis Rheum 1998 May;41(5):877-90

Date

05/20/1998

Pubmed ID

9588741

DOI

10.1002/1529-0131(199805)41:5<877::AID-ART16>3.0.CO;2-#

Scopus ID

2-s2.0-0031958104 (requires institutional sign-in at Scopus site)   96 Citations

Abstract

OBJECTIVE: To analyze the in vivo compartmental expression of collagenases 1 and 3 (MMP-1 and MMP-13) in the Hartley guinea pig model of spontaneously occurring osteoarthritis (OA) for the purpose of elucidating their roles in the pathogenesis of OA.

METHODS: Competitive reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry quantification of messenger RNA (mRNA) and protein levels in medial and lateral tibial cartilage obtained from the knee joints of 2-month-old (no OA) and 12-month-old (OA) guinea pigs.

RESULTS: The patterns of mRNA expression of collagenases 1 and 3 varied with the age of the animal and the compartment of the knee. We also found focal areas of collagenase 1 and collagenase 3 proteins localized to the extracellular matrix of OA lesion sites, coincident with three-quarter/one-quarter collagen cleavage. Collagenase 3 protein was also abundant throughout the medial tibial cartilage of 2-month-old animals.

CONCLUSION: This represents the first description of bona fide collagenase 1 in a rodent species. Recent evidence, however, based on analysis of mitochondrial DNA homologies, suggests that the guinea pig is not a member of the order Rodentia and may be more closely allied with lagomorphs. This taxonomic controversy leaves open to question the issue of the expression of collagenase 1 in other rodents, such as mice and rats. The presence of active collagenases 1 and 3 at OA lesion sites is consistent with an important role of these enzymes in the cartilage degradation of OA in guinea pigs. The expression of collagenase 3 in medial tibial cartilage from 2-month-old guinea pigs may signify a role of this enzyme in cartilage remodeling with growth and development, or it may represent an early molecular manifestation of OA.

Author List

Huebner JL, Otterness IG, Freund EM, Caterson B, Kraus VB

Author

Edward M. Nelsen-Freund MD Assistant Professor in the Orthopaedic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blotting, Southern
Collagenases
DNA Primers
Disease Models, Animal
Glyceraldehyde-3-Phosphate Dehydrogenases
Guinea Pigs
Humans
Immunohistochemistry
Male
Matrix Metalloproteinase 1
Matrix Metalloproteinase 13
Menisci, Tibial
Mice
Osteoarthritis
Polymerase Chain Reaction
RNA, Messenger
Rats
Species Specificity