Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy. Cell Rep Methods 2023 Apr 24;3(4):100453
Date
05/09/2023Pubmed ID
37159670Pubmed Central ID
PMC10163030DOI
10.1016/j.crmeth.2023.100453Scopus ID
2-s2.0-85152912837 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Visual processing in the retina depends on the collective activity of large ensembles of neurons organized in different layers. Current techniques for measuring activity of layer-specific neural ensembles rely on expensive pulsed infrared lasers to drive 2-photon activation of calcium-dependent fluorescent reporters. We present a 1-photon light-sheet imaging system that can measure the activity in hundreds of neurons in the ex vivo retina over a large field of view while presenting visual stimuli. This allows for a reliable functional classification of different retinal cell types. We also demonstrate that the system has sufficient resolution to image calcium entry at individual synaptic release sites across the axon terminals of dozens of simultaneously imaged bipolar cells. The simple design, large field of view, and fast image acquisition make this a powerful system for high-throughput and high-resolution measurements of retinal processing at a fraction of the cost of alternative approaches.
Author List
Roy S, Wang D, Rudzite AM, Perry B, Scalabrino ML, Thapa M, Gong Y, Sher A, Field GDAuthor
Miranda L. Scalabrino PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Calcium, DietaryColoring Agents
Law Enforcement
Microscopy
Neurons
