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Heat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia. Am J Physiol 1998 Jan;274(1):F215-22

Date

02/12/1998

Pubmed ID

9458842

DOI

10.1152/ajprenal.1998.274.1.F215

Scopus ID

2-s2.0-0031893041   62 Citations

Abstract

The small heat-shock proteins appear to have a regulatory role in actin dynamics. Since cytoskeletal disruption is integral to ischemic renal injury, we evaluated expression and intracellular distribution of heat-shock protein 25 (HSP-25) in rat renal cortex after 45 min of renal ischemia. HSP-25 was constitutively expressed and induced by ischemia with peak levels reached by 6 h reflow. Ischemia caused a shift of HSP-25 from the detergent-soluble into the insoluble cytoskeletal fraction. By 2 h reflow, the majority of HSP-25 had redistributed into the soluble fraction. HSP-25 was predominantly localized in a subapical distribution in control proximal tubules, a pattern intermediate between deoxyribonuclease (DNase)-reactive and filamentous actin. After ischemia, HSP-25 dispersed through the cytoplasm with small punctate accumulations similar to DNase-reactive actin. During later reflow, all three proteins were found in coarse intracytoplasmic accumulations; however, HSP-25 and DNase-reactive actin were in separate accumulations. HSP-25 and microfilamentous actin staining returned to the subapical domain. Thus the temporal and spatial patterns of HSP-25 induction and distribution suggest specific interactions between HSP-25 and actin during the early postischemic reorganization of the cytoskeleton. HSP-25 may have additional roles distinct from actin dynamics later in the course of postischemic recovery.

Author List

Aufricht C, Ardito T, Thulin G, Kashgarian M, Siegel NJ, Van Why SK

Author

Scott K. Van Why MD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Actins
Animals
Aorta, Abdominal
Cytoskeleton
Heat-Shock Proteins
Ischemia
Kidney
Kidney Tubules, Proximal
Male
Rats
Rats, Sprague-Dawley
Renal Artery