Stop-flow studies of distribution of filtration in rat lungs. J Appl Physiol (1985) 1998 Jan;84(1):47-52
Date
02/06/1998Pubmed ID
9451616DOI
10.1152/jappl.1998.84.1.47Scopus ID
2-s2.0-0031908663 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
The stop-flow approach was used to investigate where filtration occurs in the pulmonary vasculature after elevation of left atrial pressure and aspiration of HCl. Rat lungs were perfused for 11 min at zero left atrial pressures, and then flow was stopped for 10 min and left atrial pressures were increased to 20 cmH2O. Thereafter, 3HOH was instilled into the air spaces, and the pulmonary vasculature was flushed by perfusing it from the pulmonary artery to left atrium (anterograde flush) or in the opposite direction (retrograde flush). Increases in fluorescein isothiocyanate (FITC)-dextran (molecular weight 2,000,000) indicated filtration, and these preceded increases in 3HOH after anterograde but not retrograde flushes. This suggests that some filtration occurred through vessels that were relatively venous compared with those through which 3HOH exchange had occurred. Filtration increased fivefold after instillation of 0.1 N HCl in isotonic saline into the air spaces before perfusion. Increases in Evans blue-labeled albumin concentrations were < 40% those of FITC-dextran, indicating loss from the vasculature, but increases in unlabeled albumin and FITC-albumin were comparable.
Author List
Lin W, Jacobs E, Schapira RM, Presberg K, Effros RMAuthor
Kenneth W. Presberg MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Pressure
Dextrans
Endothelium, Vascular
Filtration
Fluorescein-5-isothiocyanate
Pulmonary Circulation
Pulmonary Diffusing Capacity
Pulmonary Edema
Rats
Rats, Sprague-Dawley
