High-speed imaging of evoked rodent mechanical behaviors yields variable results that are not predictive of inflammatory injury. Pain 2024 Jul 01;165(7):1569-1582
Date
02/05/2024Pubmed ID
38314814Pubmed Central ID
PMC11189758DOI
10.1097/j.pain.0000000000003174Scopus ID
2-s2.0-85196767632 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Few analgesics identified using preclinical models have successfully translated to clinical use. These translational limitations may be due to the unidimensional nature of behavioral response measures used to assess rodent nociception. Advances in high-speed videography for pain behavior allow for objective quantification of nuanced aspects of evoked paw withdrawal responses. However, whether videography-based assessments of mechanical hypersensitivity outperform traditional measurement reproducibility is unknown. First, we determined whether high-speed videography of paw withdrawal was reproducible across experimenters. Second, we examined whether this method distinguishes behavioral responses exhibited by naive mice and mice with complete Freund's adjuvant (CFA)-induced inflammation. Twelve experimenters stimulated naive C57BL/6 mice with varying mechanical stimuli. Paw withdrawal responses were recorded with high-speed videography and scored offline by one individual. Our group was unable to replicate the original findings produced by high-speed videography analysis. Surprisingly, ∼80% of variation was not accounted for by variables previously reported to distinguish between responses to innocuous and noxious stimuli (paw height, paw velocity, and pain score), or by additional variables (experimenter, time-of-day, and animal), but rather by unidentified factors. Similar high-speed videography assessments were performed in CFA- and vehicle-treated animals, and the cumulative data failed to reveal an effect of CFA injection on withdrawal as measured by high-speed videography. This study does not support using paw height, velocity, or pain score measurements from high-speed recordings to delineate behavioral responses to innocuous and noxious stimuli. Our group encourages the continued use of traditional mechanical withdrawal assessments until additional high-speed withdrawal measures are validated in established pain models.
Author List
Rodríguez García DM, Szabo A, Mikesell AR, Zorn SJ, Tsafack UK, Sriram A, Waltz TB, Enders JD, Mecca CM, Stucky CL, Sadler KEAuthors
Jonathan D. Enders Postdoctoral Researcher 1 in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinCheryl L. Stucky PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin
Aniko Szabo PhD Professor in the Data Science Institute department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBehavior, Animal
Disease Models, Animal
Freund's Adjuvant
Hyperalgesia
Inflammation
Male
Mice
Mice, Inbred C57BL
Pain Measurement
Physical Stimulation
Reproducibility of Results
Video Recording
