MUC5AC expression in human middle ear epithelium of patients with otitis media. Arch Otolaryngol Head Neck Surg 2010 Aug;136(8):819-24
Date
08/18/2010Pubmed ID
20713760Pubmed Central ID
PMC2924762DOI
10.1001/archoto.2010.123Scopus ID
2-s2.0-77955720284 (requires institutional sign-in at Scopus site) 30 CitationsAbstract
OBJECTIVE: To compare levels of middle ear (ME) MUC5AC expression in patients with otitis media (OM) with patients without OM. Mucin gene 5AC has been identified as a major secretory mucin in the ME and is fundamentally important in the development of ME mucoid effusions, hearing loss and also provides ME mucosal protection and bacterial clearance.
DESIGN: Case control.
SETTING: Tertiary, academic, pediatric otolaryngology practice.
PATIENTS: Patients 9 months to 7 years old undergoing routine tympanostomy tube (TT) insertion for recurrent otitis media (RecOM) or chronic otitis media with effusion (COME) were compared with control patients without a history of OM undergoing cochlear implantation.
METHODS: During routine TT placement or cochlear implantation, a 1-mm biopsy sample of the ME epithelium was obtained. RNA was extracted, and real-time reverse transcriptase-polymerase chain reaction was used to quantify levels of MUC5AC expression.
RESULTS: Twenty-three patients with OM (12 with RecOM and with 11 COME) were evaluated using 5 controls. Mean age was not different between groups. In the RecOM group, mean expression of MUC5AC was 25.92 times greater than in controls. In the COME group, the mean expression was 155.40 times greater than in controls.
CONCLUSIONS: Levels of MUC5AC expression in the human ME are significantly increased in patients with RecOM and COME compared with controls. This study demonstrates MUC5AC gene changes in patients with OM and highlights the need for greater understanding of the molecular responses in OM; particularly that of mucin. A thorough exploration of these factors will provide opportunities to develop novel interventions for the extremely common problem of OM.
Author List
Kerschner JE, Tripathi S, Khampang P, Papsin BCAuthor
Joseph E. Kerschner MD Provost, Executive Vice President, Dean, Professor in the School of Medicine Administration department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ChildChild, Preschool
Chronic Disease
Cochlear Implantation
Ear, Middle
Enzyme-Linked Immunosorbent Assay
Epithelium
Female
Gene Expression
Humans
Immunoenzyme Techniques
Infant
Male
Middle Ear Ventilation
Mucin 5AC
Otitis Media
Otitis Media with Effusion
Recurrence
Reference Values
Reverse Transcriptase Polymerase Chain Reaction
