Thioflavin T as a fluorescence light-up probe for G4 formation. Nucleic Acids Res 2014 Apr;42(8):e65
Date
02/11/2014Pubmed ID
24510097Pubmed Central ID
PMC4005661DOI
10.1093/nar/gku111Scopus ID
2-s2.0-84899817285 (requires institutional sign-in at Scopus site) 251 CitationsAbstract
Thioflavin T (ThT) becomes fluorescent in the presence of the G-quadruplex structure such as that formed by the human telomeric motif. In this report, we extend and generalize these observations and show that this dye may be used as a convenient and specific quadruplex probe. In the presence of most, but not all, G4-forming sequences, we observed a large increase in ThT fluorescence emission, whereas the presence of control duplexes and single strands had a more limited effect on emission. This differential behavior allowed us to design a high-throughput assay to detect G4 formation. Hundreds of different oligonucleotides may be tested in parallel for G4 formation with a simple fluorescence plate reader. We applied this technique to a family of aptamers not previously recognized as G4-forming sequences and demonstrated that ThT fluorescence signal may be used to predict G4 formation.
Author List
Renaud de la Faverie A, Guédin A, Bedrat A, Yatsunyk LA, Mergny JLAuthor
Amina Bedrat PhD Postdoctoral Fellow in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BenzothiazolesFluorescent Dyes
G-Quadruplexes
Native Polyacrylamide Gel Electrophoresis
Thiazoles
