Faculty Collaboration Database

The role of hydrogen sulfide in diastolic function restoration during aging. Fiziol Zh (1994) 2016;62(6):9-18

Date

01/01/2016

Pubmed ID

29762966

DOI

10.15407/fz62.06.009

Scopus ID

2-s2.0-85049102405 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

The objective of the study was to examine the effect of exogenous hydrogen sulde donor; sodium hydrosulfide (NaHS), on thefree radical generation, cNOS uncoupling in the myocardium, and diastolicfunction in old rats. To evaluate diastolic function of the heart, we used pressure-volume (PV) conductance catheter system (Millar Instruments, USA). It was shown that H2S levels in. the isolated mitochondria and whole heart homogenates obtained from old age rats were significantly lower comparing with adult animals. The markers of combined oxidative and nitrosative stress (the rate of 0 ₂⁰ H generation, pools of H₂0₂, diene conjugates, malondialdehyde, uric acid, the activity of iNOS, nitrate reductase, and NO pools) were increased in the old hearts in line with cNOS uncoupling. Such changes in NOS coupling resulted in the loss of diastolic relaxation (decrease of the rate of relaxation of the left ventricle (dp/dtmin) by 33%, 3-times increase of the end-diastolic pressure, 1.5-time increase of the time constant of left ventricular relaxation (Tau g) and 2-time increase of the end-diastolic stiffness). It has been found that NaHS inhibits oxidative and nitrosative stress, restores cNOS coupling and constitutive de novo synthesis of nitric oxide (NO), which promotes an improvement of the diastolic function (increase of the dp/dtmin by 20% and decrease of Tau g by 13%) . Key words: aging; cNOS uncoupling; heart; hydrogen sulfide; nitrosative stress; oxidative stress.

Author List

Drachuk KO, Dorofeyeva NA, Sagach VF

Author

Kostiantyn Drachuk Postdoctoral Fellow in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Blood Pressure
Diastole
Heart
Heart Function Tests
Hydrogen Sulfide
Male
Mitochondria, Heart
Nitric Oxide
Nitric Oxide Synthase Type I
Nitrosative Stress
Oxidative Stress
Rats, Wistar
Sulfides