Faculty Collaboration Database

[Propargylglycine restores endothelium-dependent relaxation of aortic smooth muscles in old rats]. Fiziol Zh (1994) 2014;60(4):3-10

Date

10/23/2014

Pubmed ID

25335229

DOI

10.15407/fz60.04.003

Scopus ID

2-s2.0-84921976574 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

In the study we investigated the effect of blockade cystathionine-gamma -lyase (CSE), an enzyme of hydrogen sulfide (H2S) (de novo) synthesis on the endothelium-dependent relaxation of aortic smooth muscle (SM) in old rats. It has been shown that an inhibition of CSE by propargylglycine (PAG) results in restoration of a decreased ACh-induced relaxation of aorta in old rats. This effect of PAG was removed by blocking nitric oxide (NO) synthesis in the endothelial cells. Age-related changes in the levels of H2S, NO2- and enzyme activity of the constitutive synthesis of NO (cNOS) in the heart, were determined. It has been shown that PAG introduction elevates a decreased levels of HzS, NO2- and stimulates the suppressed activity of cNOS in old rats. These results suggest that PAG activates alternative ways of H2S synthesis and stimulates the constitutive synthesis of NO. These actions of PAG restore endothelial function in old rats.

Author List

Drachuk KO, Kotsiuruba AV, Baziliuk OV, Stepanenko LH, Sahach VF

Author

Kostiantyn Drachuk Postdoctoral Fellow in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Alkynes
Animals
Aorta, Thoracic
Cystathionine gamma-Lyase
Endothelium, Vascular
Enzyme Inhibitors
Glycine
Hydrogen Sulfide
Male
Muscle, Smooth, Vascular
Nitric Oxide
Oxidative Stress
Rats
Rats, Wistar
Vasodilation