Faculty Collaboration Database

Expression Levels of lncRNAs in the Patients with the Renal Transplant Rejection. Urol J 2019 Dec 24;16(6):572-577

Date

12/15/2019

Pubmed ID

31836996

DOI

10.22037/uj.v0i0.5456

Scopus ID

2-s2.0-85077276508 (requires institutional sign-in at Scopus site)   12 Citations

Abstract

PURPOSE: Long non-coding RNAs (lncRNAs) include a vast portion of human transcripts. They exert regulatory roles in immune responses and participate in diverse biological functions. Recent studies indicated dysregulation of lncRNAs in the process of transplant rejection. In the current study, we aimed at identification of the expression of five lncRNAs (OIP5-AS1, FAS-AS1, TUG1, NEAT1 and PANDAR) in association with the process of transplant rejection.

MATERIAL AND METHODS: We assessed expression of these lncRNAs in the peripheral blood of 61 kidney transplant receivers including 29 transplant rejected patients and 32 transplant non-rejected patients using real time PCR technique.

RESULTS: Expression of FAS-AS1 was significantly higher in rejected group compared to non-rejected group in males, however, differences between case and control groups were insignificant among females. For other lncRNAs no significant differences were detected between two study groups. Quantile regression model showed that patients' gender was an important parameter in determination of FAS-AS1 expression (Beta=-9.46, t=-2.82, P=0.007) but not for other lncRNAs expressions. Significant pairwise correlations were detected between expression levels of lncRNAs in a disease related manner.

CONCLUSION: Based on the higher expression of FAS-AS1 in patients with transplant rejection, this lncRNA might be associated with the pathogenesis of renal transplant rejection.

Author List

Nafar M, Kalantari S, Ghaderian SMH, Omrani MD, Fallah H, Arsang-Jang S, Abbasi T, Samavat S, Dalili N, Taheri M, Ghafouri-Fard S

Author

Shahram Arsang-Jang Postdoctoral Fellow in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Female
Follow-Up Studies
Gene Expression Regulation
Graft Rejection
Humans
Immunity, Cellular
Kidney Failure, Chronic
Kidney Transplantation
Male
RNA, Long Noncoding
Retrospective Studies