Faculty Collaboration Database

Use of human vascular tissue microarrays for measurement of advanced glycation endproducts. J Histochem Cytochem 2009 Jun;57(6):559-66

Date

02/19/2009

Pubmed ID

19223295

Pubmed Central ID

PMC2690436

DOI

10.1369/jhc.2009.953273

Scopus ID

2-s2.0-67649229638 (requires institutional sign-in at Scopus site)   19 Citations

Abstract

Advanced glycation endproducts (AGEs) are present in the vasculature and are associated with vascular disease. We determined levels of AGEs in eight distinct adult vascular tissues using tissue microarray (TMA) technology and associated these levels with clinical characteristics. Medium-to-large caliber blood vessels were harvested from 100 adult autopsies to create 17 TMAs. AGE levels were evaluated by IHC using a polyclonal anti-AGE antibody on over 700 unique blood vessels. Slides were digitally scanned, and quantitative analysis was performed using a color deconvolution image analysis technique. Medial AGE staining was strongly correlated between all eight blood vessels. In the media, AGE staining levels were significantly higher at older ages (p=0.009), in white subjects (p<0.001) and with longer postmortem interval (PMI; p<0.0001). These associations remained significant after simultaneous adjustment for age, race/ethnicity, PMI, and diabetes status. Diabetes was associated with elevated AGE levels but only after adjustment for confounding by clinical variables including race/ethnicity, hypertension, and kidney function. This extensive vascular study shows that AGE accumulation in the macrovasculature is a global process affecting atherosclerosis-prone and -resistant vessels. It also suggests ethnicity has a previously undescribed role in vascular tissue AGE levels. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

Author List

Halushka MK, Selvin E, Lu J, Macgregor AM, Cornish TC

Author

Toby Charles Cornish MD, PhD Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Age Factors
Aged
Aged, 80 and over
Analysis of Variance
Arteries
Atherosclerosis
Diabetes Mellitus
Female
Glycation End Products, Advanced
Humans
Hypertension
Male
Microarray Analysis
Middle Aged
Sex Factors
Smoking
Tunica Intima
Tunica Media
Young Adult