Faculty Collaboration Database

Coexpression of striatal dopamine receptor subtypes and excitatory amino acid subunits. Synapse 1997 Aug;26(4):400-14

Date

08/01/1997

Pubmed ID

9215599

DOI

10.1002/(SICI)1098-2396(199708)26:4<400::AID-SYN8>3.0.CO;2-A

Scopus ID

2-s2.0-0031204172 (requires institutional sign-in at Scopus site)   49 Citations

Abstract

The striatal cellular coexpression patterns for the D(1A) and D2 dopamine (DA) receptor subtypes and the ionotropic excitatory amino acid (EAA) subunits of the N-methyl-D-aspartate (NMDA-R1) and the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) (GluR1 and GluR2/3) receptor subunits were examined morphologically. Their coincidence was assessed by visualization of mRNA transcripts, localization of encoded receptor proteins, and binding analysis using concurrently paired methods of fluorescence detection. The findings indicated that 1) mRNA transcripts for both receptor systems were detected in the medium-sized neuron population, and the distribution of receptor message closely reflected protein and binding patterns, with the exception of the GluR1 subunit; 2) both DA receptor mRNA transcripts were coexpressed with each ionotropic EAA receptor subunit examined and with each other, and NMDA and AMPA receptor subunits also showed coincident expression; 3) D(1A) DA receptor protein was detected in neurons which coexpressed EAA subunit proteins; and 4) GluR2/3 and NMDA-R1 subunit proteins were coexpressed in medium-sized neurons which also demonstrated D2 DA receptor binding sites. These findings suggest morphological receptor "promiscuity" since the coexpression patterns between DA and EAA receptors were found in all permutations. The results provide a spatial framework for physiological findings describing functional interactions between the two DA receptor types and between specific DA and EAA receptors in the striatum.

Author List

Ariano MA, Larson ER, Noblett KL, Sibley DR, Levine MS

Author

Eric Larson PhD Associate Professor in the Psychiatry and Behavioral Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Excitatory Amino Acids
Fluorescent Antibody Technique, Indirect
In Situ Hybridization, Fluorescence
Ligands
Male
Neostriatum
Neurons
Photomicrography
Rats
Rats, Sprague-Dawley
Receptors, AMPA
Receptors, Dopamine
Receptors, Dopamine D1
Receptors, Dopamine D2
Receptors, N-Methyl-D-Aspartate
Transcription, Genetic