Faculty Collaboration Database

The role of 5-HT3 and 5-HT4 receptors in the adaptive mechanism of colonic transit following the parasympathetic denervation in rats. J Surg Res 2011 Dec;171(2):510-6

Date

08/10/2010

Pubmed ID

20691988

Pubmed Central ID

PMC2978263

DOI

10.1016/j.jss.2010.05.002

Scopus ID

2-s2.0-81155128104 (requires institutional sign-in at Scopus site)   12 Citations

Abstract

BACKGROUND: Clinical studies show that disturbed colonic motility induced by extrinsic nerves damage is restored over time. We studied whether 5-HT3 and 5HT4 receptors are involved in mediating the adaptive mechanisms following parasympathetic denervation.

METHODS: Parasympathetic denervation of the entire colon was achieved by bilateral pelvic nerve transection and truncal vagotomy in rats. Colonic transit was measured by calculating the geometric center (GC) of 51Cr distribution. Expression of 5-HT3 and 5HT4 receptor mRNA was determined by real time RT-PCR.

RESULTS: Parasympathetic denervation caused a significant delay in colonic transit (GC=4.36) at postoperative day (POD) 1, compared with sham operation (GC=6.31). Delayed transit was gradually restored by POD 7 (GC=5.99) after the denervation. Restored colonic transit was antagonized by the administration of 5-HT3 and 5HT4 receptors antagonists at POD 7. 5-HT3 and 5HT4 receptors mRNA expression were significantly increased in the mucosal/submucosal layer at POD 3 or POD 7, whereas no significant difference was observed in the longitudinal muscle layers adherent with the myenteric plexus (LMMP).

CONCLUSIONS: It is suggested that up-regulation of 5-HT3 and 5-HT4 receptors expression in the mucosal/submucosal layer is involved to restore the delayed transit after the parasympathetic denervation in rats.

Author List

Tong W, Kamiyama Y, Ridolfi TJ, Zietlow A, Zheng J, Kosinski L, Ludwig K, Takahashi T

Authors

Kirk A. Ludwig MD Chief, Professor in the Surgery department at Medical College of Wisconsin
Timothy J. Ridolfi MD, MS, FACS Associate Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adaptation, Physiological
Animals
Colon
Gastrointestinal Motility
Intestinal Mucosa
Male
Myenteric Plexus
Nerve Regeneration
Parasympathectomy
Parasympathetic Nervous System
RNA, Messenger
Rats
Rats, Sprague-Dawley
Receptors, Serotonin, 5-HT3
Receptors, Serotonin, 5-HT4
Serotonin 5-HT3 Receptor Antagonists
Serotonin 5-HT4 Receptor Antagonists
Up-Regulation