Faculty Collaboration Database

Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex Multiplex technology. Clin Chem Lab Med 2005;43(12):1392-9

Date

11/29/2005

Pubmed ID

16309379

Pubmed Central ID

PMC2239010

DOI

10.1515/CCLM.2005.238

Scopus ID

2-s2.0-28644433587 (requires institutional sign-in at Scopus site)   35 Citations

Abstract

Matrix metalloproteinases are a family of zinc-dependent proteinases which are involved in the breakdown and remodeling of extracellular matrix. As children grow and adolescents reach pubescence, their bodies undergo changes that require age-related morphogenesis of the extracellular matrix, possibly requiring unique patterns of matrix metalloproteinase (MMP) expression during periods of rapid tissue growth (i.e., childhood) or accelerated tissue remodeling and expansion (i.e., adolescence). Therefore, we have characterized age-specific and gender-specific differences in circulating concentrations of MMPs (specifically MMP-1, -2, -3, -8 and -9) in 189 serum samples obtained from healthy subjects, aged 2-18 years. MMP concentrations were measured using Fluorokine MultiAnalyte Profiling kits and a Luminex Bioanalyzer, as well as by commercial ELISA. Serum levels of MMP-1, -2, -3, -8, and -9 in healthy pediatric subjects represent log-normal distributions. MMP-2 was significantly negatively correlated with age (r=-0.29; p<0.001), while MMP-3 was significantly positively correlated with age (r=0.38; p<0.001). Although plasma, not serum, is considered the appropriate blood sample for measurement of MMP-8 and -9, serum levels of MMP-8 and -9 were also found to be highly positively correlated with each other (r=0.76; p<0.01). MMP results obtained by Fluorokin MultiAnalyte Profiling methods correlated well with conventional ELISA methods and use of this technology provided several advantages over ELISA.

Author List

Thrailkill KM, Moreau CS, Cockrell G, Simpson P, Goel R, North P, Fowlkes JL, Bunn RC

Authors

Paula E. North MD, PhD Professor in the Pathology department at Medical College of Wisconsin
Pippa M. Simpson PhD Adjunct Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Biosensing Techniques
Child
Enzyme-Linked Immunosorbent Assay
Female
Humans
Luminescent Measurements
Matrix Metalloproteinase 1
Matrix Metalloproteinase 2
Matrix Metalloproteinase 3
Matrix Metalloproteinase 8
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Reference Values
Reproducibility of Results
Sensitivity and Specificity