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Initial validation of a novel protein biomarker panel for active pediatric lupus nephritis. Pediatr Res 2009 May;65(5):530-6

Date

02/17/2009

Pubmed ID

19218887

Pubmed Central ID

PMC2737257

DOI

10.1203/PDR.0b013e31819e4305

Scopus ID

2-s2.0-69049106426   78 Citations

Abstract

Lupus nephritis (LN) is among the main determinants of poor prognosis in systemic lupus erythematosus (SLE). The objective of this study was to 1) isolate and identify proteins contained in the LN urinary protein signature (PS) of children with SLE; 2) assess the usefulness of the PS proteins for detecting activity of LN over time. Using surface-enhanced or matrix-assisted laser desorption/ionization time of flight mass spectrometry, the proteins contained in the LN urinary PS were identified. They were transferrin (Tf), ceruloplasmin (Cp), alpha1-acid-glycoprotein (AGP), lipocalin-type prostaglandin-D synthetase (L-PGDS), albumin, and albumin-related fragments. Serial plasma and urine samples were analyzed using immunonephelometry or ELISA in 98 children with SLE (78% African American) and 30 controls with juvenile idiopathic arthritis. All urinary PS proteins were significantly higher with active vs. inactive LN or in patients without LN (all p < 0.005), and their combined area under the receiver operating characteristic curve was 0.85. As early as 3 mo before a clinical diagnosis of worsening LN, significant increases of urinary Tf, AGP (both p < 0.0001), and L-PGDS (p < 0.01) occurred, indicating that these PS proteins are biomarkers of LN activity and may help anticipate the future course of LN.

Author List

Suzuki M, Wiers K, Brooks EB, Greis KD, Haines K, Klein-Gitelman MS, Olson J, Onel K, O'Neil KM, Silverman ED, Tucker L, Ying J, Devarajan P, Brunner HI

Author

Judyann C. Olson MD Associate Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adolescent
Albuminuria
Arthritis, Juvenile
Biomarkers
Blood Proteins
Case-Control Studies
Ceruloplasmin
Enzyme-Linked Immunosorbent Assay
Female
Humans
Intramolecular Oxidoreductases
Lipocalins
Lupus Erythematosus, Systemic
Lupus Nephritis
Male
Nephelometry and Turbidimetry
Orosomucoid
Predictive Value of Tests
Prognosis
Proteinuria
ROC Curve
Reproducibility of Results
Serum Albumin
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
Transferrin
jenkins-FCD Prod-480 9a4deaf152b0b06dd18151814fff2e18f6c05280