Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4. Mol Cell 2002 Feb;9(2):279-89
Date
02/28/2002Pubmed ID
11864602DOI
10.1016/s1097-2765(02)00459-8Scopus ID
2-s2.0-0036184236 (requires institutional sign-in at Scopus site) 928 CitationsAbstract
The transcription factors HNF3 (FoxA) and GATA-4 are the earliest known to bind the albumin gene enhancer in liver precursor cells in embryos. To understand how they access sites in silent chromatin, we assembled nucleosome arrays containing albumin enhancer sequences and compacted them with linker histone. HNF3 and GATA-4, but not NF-1, C/EBP, and GAL4-AH, bound their sites in compacted chromatin and opened the local nucleosomal domain in the absence of ATP-dependent enzymes. The ability of HNF3 to open chromatin is mediated by a high affinity DNA binding site and by the C-terminal domain of the protein, which binds histones H3 and H4. Thus, factors that potentiate transcription in development are inherently capable of initiating chromatin opening events.
Author List
Cirillo LA, Lin FR, Cuesta I, Friedman D, Jarnik M, Zaret KSAuthor
Lisa A. Cirillo PhD Assistant Dean, Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid MotifsAnimals
Binding Sites
CCAAT-Enhancer-Binding Protein-beta
Chromatin
DNA-Binding Proteins
Endoderm
Enhancer Elements, Genetic
Fetal Proteins
GATA4 Transcription Factor
Gene Expression Regulation, Developmental
Gene Silencing
Hepatocyte Nuclear Factor 3-alpha
Histones
Liver
Macromolecular Substances
Mice
Models, Genetic
Neurofibromin 1
Nuclear Proteins
Nucleosomes
Promoter Regions, Genetic
Recombinant Fusion Proteins
Serum Albumin
Structure-Activity Relationship
Transcription Factors
Transcription, Genetic
