Faculty Collaboration Database

Assessment of CD10 in the diagnosis of small B-cell lymphomas: a multiparameter flow cytometric study. Am J Clin Pathol 2002 Feb;117(2):291-300

Date

02/28/2002

Pubmed ID

11863226

DOI

10.1309/T88X-71U4-WC0R-2531

Scopus ID

2-s2.0-0036320073 (requires institutional sign-in at Scopus site)   58 Citations

Abstract

We evaluated the usefulness of multiparameter flow cytometry with cluster analysis in the diagnosis of a series of 100 well-characterized small B-cell lymphomas (SBCLs). The histologic diagnoses in the 100 cases were follicular lymphoma (FL) in 58, marginal zone lymphoma (MZL) in 17, small lymphocytic lymphoma in 15, and mantle cell lymphoma (MCL) in 10. Of the 58 FLs, 57 were CD10 positive (98% sensitivity). The 1 negative case was unusual in that it occurred in the small intestine. However; architectural, cytologic, and immunohistochemicalfeatures were diagnostic of FL. Of 42 other SBCLs, 2 were CD10+ (95% specificity); 1 was a CD5+/cyclin D1 + MCL, and the other was an extranodal MZL. We found that assessment of CD10 expression using multiparameter flow cytometry with cluster analysis is highly sensitive and specific for the diagnosis of FL, validating its usefulness in situations in which adequate tissue is not available for definitive histologic diagnosis.

Author List

Xu Y, McKenna RW, Kroft SH

Author

Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
B-Lymphocytes
Biopsy
CD5 Antigens
Cell Lineage
Cluster Analysis
Female
Flow Cytometry
Humans
Immunoglobulin G
Immunoglobulin kappa-Chains
Immunohistochemistry
Immunophenotyping
Intestine, Small
Leukemia, Lymphocytic, Chronic, B-Cell
Lymph Nodes
Male
Middle Aged
Neprilysin
Reproducibility of Results
Sensitivity and Specificity