DNA content in nasopharyngeal carcinoma. Am J Otolaryngol 1990;11(6):393-7
Date
11/01/1990Pubmed ID
2281841DOI
10.1016/0196-0709(90)90118-fScopus ID
2-s2.0-0025597002 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
DNA analysis by flow cytometry was performed on tissue blocks from 41 patients with nasopharyngeal carcinoma. The histologic slides were reviewed by a pathologist and blindly classified according to the World Health Organization classification. The paraffin-embedded blocks were processed to obtain individual nuclei, which were then stained with propidium iodide. The nuclei were analyzed on a flow cytometer. Excluding 10 uninterpretable histograms, the remainder were interpreted blindly and classified as diploid or aneuploid. The Cox proportional hazards survival model was used to analyze stage, histology, radiation dose, and ploidy. We observed more diploids (23 of 31; 74%) than aneuploids (eight of 31; 26%). The 2-year survival rate of diploids was 55%, compared with 25% of aneuploids (P less than .05). We conclude that ploidy status is an independent prognostic factor in nasopharyngeal carcinoma.
Author List
Cheng DS, Campbell BH, Clowry LJ, Hopwood LE, Murray KJ, Toohill RJ, Hoffmann RGAuthor
Bruce H. Campbell MD Emeritus Professor in the Otolaryngology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CarcinomaCombined Modality Therapy
DNA, Neoplasm
Female
Flow Cytometry
Humans
Male
Middle Aged
Nasopharyngeal Neoplasms
Ploidies
Prognosis
Proportional Hazards Models
Survival Analysis
