Techniques for following the movement of single RNAs in living cells. Wiley Interdiscip Rev RNA 2011;2(4):601-9
Date
10/01/2011Pubmed ID
21957047DOI
10.1002/wrna.83Scopus ID
2-s2.0-84855291135 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
The ability to investigate gene expression has evolved from static approaches that analyze a population of cells to dynamic approaches that analyze individual living cells. During the last decade, a number of different fluorescent methods have been developed for monitoring the dynamics of single RNAs in living cells. Spatial-temporal analyses of single RNAs in living cells have provided novel insight into nuclear transport, RNA localization, and decay. Technical advances with these approaches allow for single molecule detection, providing an unprecedented view of RNA movement. In this article, we discuss the methods for observing single RNAs in living cells, highlighting the advantages and limitations of each method.
Author List
Urbinati CR, Long RMAuthor
Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBiological Transport, Active
Fluorescent Dyes
Gene Expression Regulation
Green Fluorescent Proteins
Humans
Microscopy, Fluorescence
RNA
Recombinant Fusion Proteins
Saccharomyces cerevisiae
