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Techniques for following the movement of single RNAs in living cells. Wiley Interdiscip Rev RNA 2011 Jul-Aug;2(4):601-9

Date

10/01/2011

Pubmed ID

21957047

DOI

10.1002/wrna.83

Scopus ID

2-s2.0-84855291135   13 Citations

Abstract

The ability to investigate gene expression has evolved from static approaches that analyze a population of cells to dynamic approaches that analyze individual living cells. During the last decade, a number of different fluorescent methods have been developed for monitoring the dynamics of single RNAs in living cells. Spatial-temporal analyses of single RNAs in living cells have provided novel insight into nuclear transport, RNA localization, and decay. Technical advances with these approaches allow for single molecule detection, providing an unprecedented view of RNA movement. In this article, we discuss the methods for observing single RNAs in living cells, highlighting the advantages and limitations of each method.

Author List

Urbinati CR, Long RM

Author

Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Biological Transport, Active
Fluorescent Dyes
Gene Expression Regulation
Green Fluorescent Proteins
Humans
Microscopy, Fluorescence
RNA
Recombinant Fusion Proteins
Saccharomyces cerevisiae