Faculty Collaboration Database

Crystal violet combined with Merocyanine 540 for the ex vivo purging of hematopoietic stem cell grafts. J Photochem Photobiol B 2003 Jul;70(3):133-44

Date

09/10/2003

Pubmed ID

12962637

DOI

10.1016/s1011-1344(03)00073-3

Scopus ID

2-s2.0-0141792990 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

The purpose of this study was to determine in a preclinical purging model, how effective crystal violet-mediated photodynamic therapy (CV-PDT) is against solid tumor and drug-resistant mutant tumor cells, and if certain limitations of CV-PDT can be overcome by using crystal violet (CV) in combination with the membrane-active photosensitizer, Merocyanine 540 (MC540). When used under conditions that preserved an adequate fraction of normal human granulocyte/macrophage progenitors (CFU-GM), CV-PDT failed to achieve meaningful reductions of DU145 prostate, H69 small cell lung cancer, and MDA-MB-435S breast cancer cells. Melphalan-resistant L1210/L-PAM1, adriamycin-resistant P388/ADR, and adriamycin-resistant HL-60/ADR leukemia cells were markedly less sensitive to CV-PDT than their wild-type counterparts, whereas cisplatin-resistant H69/CDDP cells were more sensitive than wild-type H69 cells. Sequential exposure to MC540- and CV-PDT under conditions that preserved an adequate fraction (73% and 29%, respectively) of normal CD34-positive hematopoietic stem cells and granulocyte/macrophage progenitors was highly effective against H69 (99.997% reduction) and H69/CDDP (99.999% reduction) cells, but ineffective against HL-60/ADR, MDA-MB-435S, and DU145 cells. CV thus shows only limited promise as a single-modality purging agent. However, in certain situations, clinically meaningful tumor cell depletions can be obtained by using CV in combination with a second photosensitizer such as MC540.

Author List

Miyagi K, Sampson RW, Sieber-Blum M, Sieber F

Author

Fritz Sieber PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Bone Marrow Purging
Caspases
Cell Line, Tumor
Cell Survival
Drug Resistance, Neoplasm
Gentian Violet
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Mice
Molecular Structure
Necrosis
Neoplasms
Photochemotherapy
Photosensitizing Agents
Pyrimidinones
Receptors, Peptide