Faculty Collaboration Database

Comparative evaluation of a new immunoradiometric assay for corticotropin. Clin Chem Lab Med 2006;44(5):669-71

Date

05/10/2006

Pubmed ID

16681443

DOI

10.1515/CCLM.2006.113

Scopus ID

2-s2.0-33646699125 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

We have characterized the performance of a commercial two-site immunoradiometric assay for manual in vitro diagnostic measurement of plasma corticotropin from Scantibodies Laboratory. We compared the results with those of a similar commonly used assay from Nichols Institute Diagnostics that has recently been withdrawn from production. The lower detection limit, range of the standard curve, cross-reactivity, and intra-assay and inter-assay imprecision of the two assays are very similar. Measurement of clinical samples and a series of samples from an experimental subject demonstrate high correlations between the two assays. These factors, together with recent clearance by the United States Food and Drug Administration for manual in vitro diagnostic measurement, make the Scantibodies corticotropin immunoradiometric assay an appropriate replacement for the Nichols assay.

Author List

Wilkinson CW, Raff H

Author

Hershel Raff PhD Professor in the Academic Affairs department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adrenocorticotropic Hormone
Chemistry, Clinical
Dose-Response Relationship, Drug
Evaluation Studies as Topic
Humans
Immunoradiometric Assay
Regression Analysis
Reproducibility of Results
Sensitivity and Specificity
Time Factors