Endotoxin-induced reduction in biliary indocyanine green excretion rate in a chronically catheterized rat model. Am J Physiol Gastrointest Liver Physiol 2001 May;280(5):G858-65
Date
04/09/2001Pubmed ID
11292593DOI
10.1152/ajpgi.2001.280.5.G858Scopus ID
2-s2.0-0035026231 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Using a nonstressed chronically catheterized rat model in which the common bile duct was cannulated, we studied endotoxin-induced alterations in hepatic function by measuring changes in the maximal steady-state biliary excretion rate of the anionic dye indocyanine green (ICG). Biliary excretion of ICG was calculated from direct measurements of biliary ICG concentrations and the bile flow rate during a continuous vascular infusion of ICG. Despite significant elevations in mean peak serum tumor necrosis factor-alpha (TNF-alpha) concentrations (90.9 +/- 16.2 ng/ml), there was no effect on mean rates of bile flow or biliary ICG clearance after administration of 100 microg/kg endotoxin at 6 or 24 h. Significant differences from mean baseline rates of bile flow and biliary ICG excretion did occur after administration of 1,000 microg/kg endotoxin (mean peak TNF-alpha 129.6 +/- 24.4 ng/ml). Furthermore, when rats were treated with up to 16 microg/kg of recombinant TNF-alpha, there was no change in mean rates of bile flow or ICG biliary clearance compared with baseline values. These data suggest that the complex regulation of biliary excretion is not mediated solely by TNF-alpha.
Author List
Beno DW, Uhing MR, Goto M, Chen Y, Jiyamapa-Serna VA, Kimura REAuthor
Michael R. Uhing MD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBile
Catheters, Indwelling
Common Bile Duct
Corticosterone
Endotoxins
Escherichia coli
Gallbladder
Indocyanine Green
Kinetics
Lipopolysaccharides
Liver
Male
Models, Animal
Rats
Rats, Sprague-Dawley
Recombinant Proteins
Sepsis
Time Factors
Tumor Necrosis Factor-alpha
