Large cell lymphoma transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma. A flow cytometric analysis of seven cases. Am J Clin Pathol 2001 Mar;115(3):385-95
Date
03/13/2001Pubmed ID
11242795DOI
10.1309/F5WE-K2T8-BT9D-Q56DScopus ID
2-s2.0-0035292737 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
We studied 7 cases of large cell transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) immunophenotyped by multiparameter flow cytometry. The 6 women and 1 man ranged in age from 45 to 91 years. All had previous or concurrent evidence of CLL/SLL. Morphologic features and sites of involvement of the diffuse large B-cell lymphoma (DLBCL) were heterogeneous; 2 cases had paraimmunoblastic morphologic features. Six DLBCLs had an immunophenotype consistent with CLL: CD19+, CD5+, CD23+, and FMC7 negative (3 cases) or very dim (2 cases); 1 case was not studied for FMC7. CD20 was dim in 3 of these, moderate to bright in 2, and variable in 1. Surface immunoglobulin was dim in 2 cases and moderate or bright in 4. Five of 6 expressed CD38. Comparison with the immunophenotypes of the previous or coexistent CLL/SLL (4 of 6 cases) revealed minor modulations in antigen expression but no major alterations. The seventh DLBCL lacked CD5 expression, but otherwise had immunophenotypic features similar to CLL. These findings indicate that DLBCL arising in CLL/SLL tends to retain a CLL immunophenotype, in contrast with de novo CD5+ large cell lymphomas that uncommonly express such a phenotype.
Author List
Kroft SH, Dawson DB, McKenna RWAuthor
Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ADP-ribosyl CyclaseADP-ribosyl Cyclase 1
Aged
Aged, 80 and over
Antigens, CD
Antigens, CD19
Antigens, CD20
Antigens, Differentiation
Bone Marrow
CD5 Antigens
Cell Nucleus
Cytoplasm
Female
Flow Cytometry
Glycoproteins
Humans
Immunophenotyping
Leukemia, Lymphocytic, Chronic, B-Cell
Lymphoma, B-Cell
Lymphoma, Large B-Cell, Diffuse
Male
Membrane Glycoproteins
Middle Aged
NAD+ Nucleosidase
Receptors, IgE
Time Factors
