Faculty Collaboration Database

Cadmium inhibits glucose uptake in primary cultures of mouse cortical tubule cells. Am J Physiol 1990 Jun;258(6 Pt 2):F1625-33

Date

06/01/1990

Pubmed ID

2360656

DOI

10.1152/ajprenal.1990.258.6.F1625

Scopus ID

2-s2.0-0025369670 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

We studied the effect of cadmium (Cd2+) on transport of alpha-methylglucoside in primary cultures of mouse kidney cortical tubule cells grown in defined medium. When cultured cells were exposed to Cd2+ concentrations from 0 to 6 microM for 24 h, uptake of alpha-methylglucoside was inhibited in a dose-dependent manner by up to 50%. By contrast, acute exposure of the cells to 7 microM Cd2+ for 60 min did not inhibit alpha-methylglucoside uptake. Increasing Cd2+ concentrations progressively decreased the Vmax of Na(+)-dependent glucose cotransport but not the Km for glucose. Cell ATP/ADP ratios of unexposed monolayers and of cells exposed to 4.5 microM Cd2+ for 24 h were 5.0 and 4.9, respectively (n = 3). Intracellular volume, lactate dehydrogenase activity, and cell Na+ and K+ concentrations were unaltered even after 24 h of exposure to 7 microM Cd2+. Untreated and Cd2+-treated monolayers preloaded with alpha-methylglucoside released the sugar analogue into the medium at nearly identical rates, indicating that Cd2+ did not alter cell permeability to glucose. Uptake of the amino acid analogue alpha-(methylamino)isobutyric acid was not affected by prior Cd2+ exposure. Whereas cell DNA content declined in Cd2(+)-exposed plates, both Na(+)-glucose and Na(+)-amino acid cotransport were enhanced at lower cell densities. Protein and DNA synthesis, estimated, respectively, by incorporation of [3H]leucine and [3H]thymidine into acid-insoluble material, were not significantly affected at 6 microM Cd2+. We conclude that after a lag time Cd2+ selectively inhibits renal Na(+)-dependent glucose transport despite an unchanged gradient for Na+ across the cell membrane.

Author List

Blumenthal SS, Lewand DL, Buday MA, Kleinman JG, Krezoski SK, Petering DH

Author

Samuel S. Blumenthal MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aminoisobutyric Acids
Animals
Biological Transport
Cadmium
Cell Membrane Permeability
Cells, Cultured
Glucose
Kidney Cortex
Kidney Tubules
L-Lactate Dehydrogenase
Methylglucosides
Potassium
Sodium
Water