Faculty Collaboration Database

Splenectomy ablates endotoxin-induced IFNgamma response in rats. Shock 2002 Apr;17(4):312-5

Date

04/17/2002

Pubmed ID

11954833

DOI

10.1097/00024382-200204000-00013

Scopus ID

2-s2.0-0036546350 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

The mechanism of liver injury in endotoxemia is unclear. Previous studies have shown that splenectomy protects the liver from endotoxin-induced injury. The purpose of this study was to determine the relationship of TNFalpha and IFNgamma release and endotoxin-induced liver injury in splenectomized and nonsplenectomized rats. Splenectomized and nonsplenectomized (Sham) rats with chronic catheters in the aorta and inferior vena cava (IVC) were parenterally infused with 10 to 5000 microg/kg endotoxin. TNFalpha, IFNgamma, and alanine aminotransferase (ALT), a marker of hepatocellular damage, were measured in aortic blood. Compared to sham controls, splenectomized animals demonstrated significantly reduced endotoxin-induced ALT concentrations at endotoxin doses >10 microg/kg. Peak endotoxin-induced TNFalpha concentrations were not significantly different between the splenectomized and sham groups. In contrast, peak endotoxin-induced IFNgamma concentrations were significantly decreased in the splenectomized group. These data suggest a relationship between endotoxin-induced IFNgamma and liver injury. We speculate that the spleen contributes to the endotoxin-induced liver injury by modulating release of IFNgamma.

Author List

Deriy LV, Beno DW, Uhing MR, Jiyamapa-Serna VA, Kimura RE

Author

Michael R. Uhing MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Alanine Transaminase
Animals
Endotoxins
Interferon-gamma
Liver
Male
Rats
Rats, Sprague-Dawley
Spleen
Splenectomy
Tumor Necrosis Factor-alpha