Medical College of Wisconsin
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Comparative analysis of light chain expression in germinal center cells and mantle cells of reactive lymphoid tissues. A four-color flow cytometric study. Am J Clin Pathol 2003 Jan;119(1):130-6

Date

01/11/2003

Pubmed ID

12520708

DOI

10.1309/9MYM-D68F-U8YE-843D

Scopus ID

2-s2.0-85047688422 (requires institutional sign-in at Scopus site)   27 Citations

Abstract

We studied skewing of light chain ratios (LCRs) in germinal center cells (GCCs) relative to mantle cells (MCs) by flow cytometry (FC) in 98 reactive lymphoid tissues. LCRs were assessed using a 4-color lambda/kappa/CD20/CD38 tube. GCCs and MCs were discriminated by CD20 and CD38 density. Of 98 cases, 65 contained distinct GCCs and MCs. Light chain expression usually was dimmer on GCCs than on MCs; in 22 cases, the kappa and lambda clusters converged and accurate LCRs could not be determined. Of the remaining 43 cases, the mean GCC LCR was 1.78 (range, 1.10-3.07) vs 1.56 (range, 1.00-2.24) in the MCs (P = .001). The overall kappa/lambda ratio in cases containing GCCs and MCs was 1.65 (range, 1.18-2.69) compared with 1.46 (range, 1.00-1.98) in cases containing MCs only. Of 43 cases, 19 (44%) showed differences of 20% or more between the LCRs of GCCs and MCs. LCRs of GCCs and MCs may differ substantially in reactive lymphoid tissues. These subsets may form distinct clusters and skews in their LCRs and should not be misinterpreted as evidence of occult lymphoma.

Author List

Reichard KK, McKenna RW, Kroft SH

Author

Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Aged
B-Lymphocytes
Cell Separation
Child
Child, Preschool
Female
Flow Cytometry
Germinal Center
Humans
Immunoglobulin kappa-Chains
Immunoglobulin lambda-Chains
Immunophenotyping
Infant
Male
Middle Aged
Pseudolymphoma