Comparative analysis of light chain expression in germinal center cells and mantle cells of reactive lymphoid tissues. A four-color flow cytometric study. Am J Clin Pathol 2003 Jan;119(1):130-6
Date
01/11/2003Pubmed ID
12520708DOI
10.1309/9MYM-D68F-U8YE-843DScopus ID
2-s2.0-85047688422 (requires institutional sign-in at Scopus site) 27 CitationsAbstract
We studied skewing of light chain ratios (LCRs) in germinal center cells (GCCs) relative to mantle cells (MCs) by flow cytometry (FC) in 98 reactive lymphoid tissues. LCRs were assessed using a 4-color lambda/kappa/CD20/CD38 tube. GCCs and MCs were discriminated by CD20 and CD38 density. Of 98 cases, 65 contained distinct GCCs and MCs. Light chain expression usually was dimmer on GCCs than on MCs; in 22 cases, the kappa and lambda clusters converged and accurate LCRs could not be determined. Of the remaining 43 cases, the mean GCC LCR was 1.78 (range, 1.10-3.07) vs 1.56 (range, 1.00-2.24) in the MCs (P = .001). The overall kappa/lambda ratio in cases containing GCCs and MCs was 1.65 (range, 1.18-2.69) compared with 1.46 (range, 1.00-1.98) in cases containing MCs only. Of 43 cases, 19 (44%) showed differences of 20% or more between the LCRs of GCCs and MCs. LCRs of GCCs and MCs may differ substantially in reactive lymphoid tissues. These subsets may form distinct clusters and skews in their LCRs and should not be misinterpreted as evidence of occult lymphoma.
Author List
Reichard KK, McKenna RW, Kroft SHAuthor
Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Aged
B-Lymphocytes
Cell Separation
Child
Child, Preschool
Female
Flow Cytometry
Germinal Center
Humans
Immunoglobulin kappa-Chains
Immunoglobulin lambda-Chains
Immunophenotyping
Infant
Male
Middle Aged
Pseudolymphoma