Faculty Collaboration Database

Reactive oxygen species are critical mediators of coronary collateral development in a canine model. Am J Physiol Heart Circ Physiol 2003 Oct;285(4):H1582-9

Date

06/21/2003

Pubmed ID

12816750

DOI

10.1152/ajpheart.00318.2003

Scopus ID

2-s2.0-0141676778 (requires institutional sign-in at Scopus site)   48 Citations

Abstract

Recent evidence suggests that reactive oxygen species (ROS) promote proliferation and migration of vascular smooth muscle (VSMC) and endothelial cells (EC). We tested the hypothesis that ROS serve as crucial messengers during coronary collateral development. Dogs were subjected to brief (2 min), repetitive coronary artery occlusions (1/h, 8/day, 21 day duration) in the absence (occlusion, n = 8) or presence of N-acetylcysteine (NAC) (occlusion + NAC, n = 8). A sham group (n = 8) was instrumented identically but received no occlusions. In separate experiments, ROS generation after a single 2-min coronary artery occlusion was assessed with dihydroethidium fluorescence. Coronary collateral blood flow (expressed as a percentage of normal zone flow) was significantly increased (71 +/- 7%) in occlusion dogs after 21 days but remained unchanged (13 +/- 3%) in sham dogs. Treatment with NAC attenuated increases in collateral blood flow (28 +/- 8%). Brief coronary artery occlusion and reperfusion caused ROS production (256 +/- 33% of baseline values), which was abolished with NAC (104 +/- 12%). Myocardial interstitial fluid produced tube formation and proliferation of VSMC and EC in occlusion but not in NAC-treated or sham dogs. The results indicate that ROS are critical for the development of the coronary collateral circulation.

Author List

Gu W, Weihrauch D, Tanaka K, Tessmer JP, Pagel PS, Kersten JR, Chilian WM, Warltier DC

Author

Dorothee Weihrauch DVM, PhD Research Scientist II in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Division
Collateral Circulation
Coronary Circulation
Coronary Disease
Coronary Vessels
Dogs
Endothelial Growth Factors
Endothelium, Vascular
Extracellular Space
Intercellular Signaling Peptides and Proteins
Lymphokines
Muscle, Smooth, Vascular
Myocardium
Neovascularization, Pathologic
Reactive Oxygen Species
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors