Faculty Collaboration Database

Lack of effect of beta-naphthoflavone on induction of Nramp genes in adult rainbow trout Oncorhynchus mykiss. Mar Environ Res 2000;50(1-5):147-51

Date

07/20/2001

Pubmed ID

11460681

DOI

10.1016/s0141-1136(00)00113-6

Scopus ID

2-s2.0-0033755984 (requires institutional sign-in at Scopus site)

Abstract

Natural resistance-associated macrophage protein (Nramp) genes in rainbow trout, Oncorhynchus mykiss, were identified and characterized. The greatest mRNA level encoding these genes was in the developing ovary of rainbow trout. We evaluated the response of these genes to a certain aromatic hydrocarbon receptor (AHR) agonist. Adult rainbow trout were treated with beta-naphthoflavone (BNF) (50 and 100 mg/kg) for 48 h. Using reverse-transcriptase polymerase chain reaction with ovary and head kidney RNA and specific alpha and beta Nramp primers, a 400 bp Nramp-alpha- and a 400 bp Nramp-beta-specific cDNA were obtained. There were no changes in the alpha and beta Nramp mRNA levels in the ovary following BNF administration. CYP1A1 mRNA was increased in the ovary and kidney, suggesting the presence of AHR in rainbow trout ovary, while the AHR agonist produced no effect on Nramp mRNAs.

Author List

Dasmahapatra AK, Wimpee BA, Budsberg KJ, Dorschner MO, Phillips RB, Hutz RJ

Author

Reinhold J. Hutz in the Biological Sciences department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

Animals
Carrier Proteins
Cation Transport Proteins
Cytochrome P-450 CYP1A1
Female
Gene Expression Regulation
Iron-Binding Proteins
Kidney
Male
Membrane Proteins
Oncorhynchus mykiss
Ovary
RNA, Messenger
Receptors, Aryl Hydrocarbon
Reverse Transcriptase Polymerase Chain Reaction
Water Pollutants, Chemical
beta-Naphthoflavone