Faculty Collaboration Database

Nonhuman primate in vitro fertilization: biochemical changes associated with embryonic development. Fertil Steril 1983 Oct;40(4):521-4

Date

10/01/1983

Pubmed ID

6617915

Scopus ID

2-s2.0-0021037586 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

Little research has focused on the biochemistry of primate embryos fertilized in vitro. These studies were designed to examine alterations in cellular incorporation of leucine, uptake of steroid hormones, and overall viability of such embryos. The results may be applied to certain investigations of embryonic development in humans. Squirrel monkey ova and embryos were incubated in medium supplemented with either 3H-leucine, 3H-17 beta-estradiol (E2), or 3H-progesterone (P) and processed for autoradiographic analysis or assayed for radioactivity. Cellular incorporation of 3H-leucine (as an indication of protein synthesis), declined with oocyte maturation in vitro and remained constant at in vitro fertilization (IVF). The use of the vital dyes trypan blue and fluorescein diacetate showed high correlations with the cellular incorporation of 3H-leucine by ova, therefore demonstrating viability. Uptake of E2 and P increased at IVF. The results indicate normal metabolic development of primate embryos fertilized in vitro that is comparable to preimplantation development of other mammalian species.

Author List

Hutz RJ, Chan PJ, Dukelow WR

Author

Reinhold J. Hutz in the Biological Sciences department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

Animals
Autoradiography
Estradiol
Female
Fertilization in Vitro
Fluoresceins
Leucine
Male
Oocytes
Progesterone
Saimiri
Trypan Blue